WebBoth dextran- and Ficoll-based seeding methods supported human mesenchymal stromal cells viability and osteogenic differentiation post-seeding. Interestingly, the improved cell distribution led to increased matrix ... Density and viscosity measurements of Dextran and Ficoll based solutions Ficoll-Paque™ Plus solution (GE Healthcare) was ... WebWe claim:1. In a process for the purification of a biologically active substance with the aid of a system of at least two immiscible aqueous phases, the improvement that said substance is caused to bind to insoluble particles which have an affinity for said substance and distribute predominantly quantitative into one of said phases, and after separating the particle …
MANUFACTURE OF CHROMATOGRAPHY MATRICES - GE …
WebDextran is a synthetic colloid consisting of mixture of glucose polymers derived from the action of Leuconostoc mesenteroides on sucrose and is currently available in 10% dextran 40 (40 kDa) and 6% dextran 70 (70 kDa). Dextrans have high water-binding capacity; for example, 1 g of dextran 40 retains 30 mL of water, while 1 g dextran 70 retains ... WebDextran, a high-molecular-weight poly-D-glucose, and ficoll, a synthetic polymer of epichlorhydrin and sucrose, are electroneutral, hydrophilic polysaccharides whose size can be varied. This variability in size coupled with their membrane impermeability can make them useful for studying fluid-phase pinocytosis, the size of membrane pores (e.g ... in a well-balanced manner
FITC-labelled polysaccharides - Sigma-Aldrich
WebHrčak portal hrvatskih znanstvenih i stručnih časopisa - Hrčak WebJul 14, 2016 · The Ficoll approach resulted in the lowest cell recovery with an average of 6 x 10 5 cells/ml of whole blood. The SepMate tubes and CPTs resulted in cell recovery of 8 x 10 5 cells/ml and 13 x 10 5 cells/ml … WebJan 26, 1987 · Preservation of function by cells prepared by the Hypaque-Ficoll method have also been shown to compare favourably with that of cells prepared by other techniques such as Dextran sedimentation followed by hypotonic lysis of contaminating erythrocytes (Hokland and Berg, 1981; Bignold, unpublished results). inappropriate touch social story