WebNRF2/ARE Responsive Luciferase Reporter HEK293 Cell Line is derived from Human embryonic kidney, and stably express firefly luciferase reporter gene under the control of NRF2/ARE response element. This cell line is an ideal cellular model for monitoring the activation of Antioxidant response Pathway triggered by stimuli treatment, enforced gene … Web31 jul. 2014 · The proteasome cleaves intracellular proteins into peptides. Earlier studies found that treatment of human embryonic kidney 293T (HEK293T) cells with epoxomicin (an irreversible proteasome inhibitor) generally caused a decrease in levels of intracellular peptides. However, bortezomib (an antitumor d …
NRF2, NFE2L2 antibody (80593-1-RR) Proteintech - ptglab
Web21 feb. 2024 · Stable HEK293-Nrf2 Cells Expressed rFVII Higher Than HEK293 Cells. Next to investigate whether establishment of Nrf2-overexpressing HEK293 cells influence the expression level of any recombinant protein, the stable HEK-Nrf2 (C1) and HEK-Nrf2 (C2) cells and the stable HEK-V cells were transfected with pcDNA4B-FVII construct … Web20 feb. 2024 · The pTRAF allowed determination of Nrf2, HIF, and NF-κB activities in a high-resolution and high-throughput manner, and we here assessed how redox … dmg\u0027s s. r. o
The protective role of Nrf2-Gadd45b against antimony-induced
WebNRF2 antibody detects NRF2 protein by western blot analysis. Non-transfected (-) and NRF2-transfected (+, including 3xFlag-tag) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody (GTX103322) diluted by 1:1000. Web12 feb. 2015 · LH601A is a first-in-class direct inhibitor of the KEAP1-NRF2 protein–protein interaction. The purpose of this study was to determine whether LH601A activates NRF2 signaling in human kidney cells. Human embryonic kidney 293 (HEK293) cells were treated with LH601A or the indirect NRF2 activator, sulforaphane (SFN) for 6 or 16 h. WebHepa-1, HepG2, HEK293, and FRT-FLAG-Nrf2 293 cells were plated at a density of 2000 cells/well in 96-well plates. After 20 h, Hepa-1 and HepG2 cells were treated with DMSO or t-BHQ, and HEK293 and FRT-Nrf2 293 cells were … dmgt take private