Phis2.1 vector

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August undefined, 2024

Webb6 sep. 2024 · 1）pHis2-bait和AD-prey质粒混合作为实验组，将pHis2-bait和空载AD质粒混合作为自激活组。将实验组和自激活组分别共转到Y187感受态细胞中。 2）用SD/-Leu/ … Webb16 feb. 2024 · Two GA-inducible genes expansin 1 (PhEXP1) and cysteine proteinase 1 (PhCP1) were down-regulated in the flowers infected with TRV-PhCHS/GA20ox3 . Expression levels of PhSAG12 and PhSAG29 in TRV- PhCHS / GA20ox3 -infected corollas were detected to be significantly higher than those in the corollas infected with the …

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WebbPlasmid Phis2 1 Cloning Reporter Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article … http://www.biofeng.com/zaiti/jiaomuzajiao/pHis2.html chris sprowls speaker of the house

OsGRAS23, a rice GRAS transcription factor gene, is involved in …

http://www.biofeng.com/zaiti/jiaomu/pYES2.html WebbThe target DNA sequence, or Bait Sequence, is cloned into pHIS2.1 as one copy or tandem repeats. Interaction between a DNA-binding protein and the target sequence stimulates … WebbVectors in Use. N or C-terminal His tag. Parallel vectors 1 & 3 contain N-terminal His (pET22), MBP (pMal) and GST (pGex) with similar MCS for easy cloning. Parallel vector 2 contains N and C-terminal His (pET22), … chris spurgeon

Construction of yeast one-hybrid library for screening suspensor ...

RNA-Seq Analysis Identifies Transcription Factors Involved in ...

WebbpYES2的是一个5.9 kb的载体，设计用来在酿酒酵母（Saccharomyces cerevisiae）中诱导表达重组蛋白。. 载体的特点在于基因插入载体的构建简单，以及能够使用原养型尿嘧啶进 … Webb9 apr. 2024 · The recombinant vectors were transferred into yeast strain Y187 cells using the LiAC-PEG4000 method. The TF-encoding genes were amplified and inserted into the pGADT7 vector, and the resulting constructs were then transformed into the pHis2 yeast strain containing the promoter plasmid. chris sprowls speaker of the florida houseWebbClose. We will increase some of our prices on April 1, 2024. Be sure to complete your order before this time to take advantage of current prices. See the new prices and get more … chris sprowls wife

"Webb24 jan. 2024 · For ligation: the molar ratio of your bait sequence: vector ranges from 3:1 and 5:1. The reaction can be put at 16 degrees overnight (in PCR cycler machine). It … " - Phis2.1 vector

Phis2.1 vector

pGreenII 0800-LUC (VET1290) - Creative Biogene

Webb16 okt. 2014 · Specifically, the bait construct pHis2.1−2 × 150 was generated by cloning tandem copies of the 150-bp enhancer (2 × 150) into the MluI site of pHis2.1, a one … Webb19 sep. 2024 · C-repeat binding factors/dehydration responsive element binding factors 1 (CBFs/DREB1s) are a small family of transcription factors that play important roles in …

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http://www.yrgene.com/documents/vector/matchmaker_library_constructionscreening_kits_user_manual.pdf WebbCentrifuge at 5,000×g for 5 min. 2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. …

WebbpTALEN v2 (NN) pTALEN v2 (HD) pX335 pX260 pX320 pShuttle-CMV pShuttle pAdTrack-CMV pAdTrack pAdEasy-1 BJ5183 AdEasy-1 pAd/BLOCK-iT-DEST RNAi Gateway Vector pDC315 pBHGloxdelE13cre pShuttle-IRES-hrGFP2 pAAV-MCS pAAV-RC2 pAAV-RC pHelper pAAV-LacZ pAAV-IRES-hrGFP pCMV-MCS RNAi-Ready pSIREN-RetroQ RNAi-Ready … WebbIn this construct, three copies of the DNA target (T) have been inserted into the pHIS2.1 reporter vector. GAL UAS minimal promoterADE2, HIS3, lacZ, and MEL1 transcription …

Webb16 jan. 2015 · (PR732189;publishedApril2007)pHIS2.1VectorInformationPT3951-5Sold630304RestrictionMapMultipleCloningSite(MCS)pHIS2.1Vector.Uniquerestrictionsitesbold.DescriptionpHIS2.1reportervectoryeastone … Webb27 mars 2024 · 筛选含有报道质粒的酵母细胞具体过程为：合成靶序列；将靶序列插入pHIS2载体的多克隆位点；一般为将0.1mg pHIS2质粒用EcoRⅠ和SacⅠ进行完全双酶 …

Webb5 juli 2024 · The empty vector pHIS2.1 was used as the negative control. All cells carrying different vector combinations grew normally and equally on the SD/-Leu/-Trp medium (Fig. 4 b). However, the growth of yeast cells with the combination of pGADT7/pHIS2.1 empty vectors or pGADT7- GsERF71 /pHIS2.1 vectors was significantly inhibited on the SD/ …

WebbLower panel revealed that pHis2.1 vector encompassing FL, ∆1, or ∆2 promoter region of the ANAC003 gene was amplified, respectively by using the primer set located at the … chris spurioWebbChina Agricultural University (CAU, Chinese: 中国农业大学; pinyin: Zhōngguó Nóngyè Dàxué; abbreviated 农大/Nóngdà) is a university in Beijing, People's Republic of China … chris spurginWebb联系人：Dr.Xu, Biovector NTCC Inc. 电话： 400-800-2947 工作QQ:1843439339 (微信同号) 邮件： [email protected]. 手机： 18901268599. 地址：北京. 已注册. 详细信息. 咨询 … geological outcrophttp://www.youbio.cn/product/vt1637 geological park meaninghttp://www.qualityard.com/upload/2024-12-17/dbd8dacfb1f8d0626e1c10bc4840ba05.pdf geological period of time crosswordWebb19 sep. 2024 · The pHIS2 vector carries the TRP1 nutritional marker for selection in yeast. The transformed yeast cells were cultured on SD/-Trp-Leu medium and SD/-Trp-His-Leu medium supplemented with 10 mM or 30 mM 3-amino … chris spurio cbizWebbChina Agricultural University (CAU, Chinese: 中国农业大学; pinyin: Zhōngguó Nóngyè Dàxué; abbreviated 农大/Nóngdà) is a university in Beijing, People's Republic of China specializing in agriculture, biology, engineering, veterinary medicine, economics, management, humanities and social science. geological period noted for evolution of fish