Phosphomotif antibodies

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August undefined, 2024

WebPhosphoproteomic screen for PDK4 substrates, followed by nonphosphorylatable and phosphomimetic mutations of the PDK4 site revealed cytoplasmic GTPase, Septin 2 (SEPT2), as the key effector … WebJan 1, 2005 · The antibody reactive with the phosphomotif generated by Akt, referred to here as the PAS (phospho Akt substrate) antibody, was purchased from Cell Signaling Technology, Beverly, MA (catalog number 9611). Rabbit antisera against peptides from the insulin-elicited phosphoproteins were generated for us by BioSource International, …

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WebThis study describes a method for the identification of the substrates of specific serine kinases. An antibody specific for the phosphomotif generated by the kinase is used to … WebPrimary Antibodies (9010) Secondary Antibodies (76) Antibody Conjugates (1994) Activators & Inhibitors (224) Buffers & Dyes (108) Cellular Assay Kits (118) ChIP Kits & Reagents (156) CUT&RUN Kits & Reagents (15) Cytokines & Growth Factors (215) ELISA Kits (513) Experimental Controls (248) Flow Cytometry Kits & Reagents (9) Proteomic Analysis … incolink number

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WebPhosphosite-specific antibodies can be produced as polyclonals or monoclonals in different animal species, and each approach offers its own benefits and disadvantages. The … WebJan 4, 2013 · These antibodies are commonly used in immunoprecipitation and/or immunoblotting protocols to determine kinase-induced substrate phosphorylation. This … WebApr 13, 2024 · Figure 1.Expression levels of obscurin in wild-type and Obscn-ΔIg58/59 atria.(A,A’) Immunoblotting (A) and relative quantification (A’) of giant obscurin in wild-type 12-month-old left ventricles (LV) and atrial lysates utilizing antibodies to Ig67 did not reveal significant expression differences; t-test, p = 0.18.(B) Immunoblotting of giant obscurin in … incolink pds

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Phosphomotif antibodies

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WebPhosphoMotif Finder is especially useful for researchers who identify phosphorylation sites and would like to know if there is any specific enzyme associated with this function. The … WebJan 9, 2014 · After 2D-DIGE analysis, changed protein spots were characterized for reactivity to ERK phosphomotif antibodies and identified by MS. Various types of phosphomotif …

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WebDownload scientific diagram Phosphomotif antibody 5557 is phosphospecific and detects MAPK phosphorylation events. A, sequence of phosphopeptide library containing two … WebDec 8, 2015 · Together, using a TNIK inhibitor and phosphomotif antibody, we identify endogenous substrates of TNIK in neurons, define consensus sequences for TNIK, and suggest signaling pathways by which...

WebFeb 1, 2016 · Together, using a TNIK inhibitor and phosphomotif antibody, we identify endogenous substrates of TNIK in neurons, define consensus sequences for TNIK, and … Web2024年4月の新製品リストです。

WebUnlike phosphoantibodies raised against a single amino acid sequence, phosphomotif antibodies (first developed by Cell Signaling Technology) recognize many different phosphoproteins, ... WebPhosphospecific antibodies are affinity-purified rabbit polyclonal or monoclonal antibodies that are monospecific for a target protein that is phosphorylated. These antibodies …

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WebThe validation of phosphosite-specific antibodies requires multiple techniques and tactics to demonstrate their specificity. These antibodies can be used in arrays, flow cytometry, and … incolink softwareWebFeb 11, 2013 · The scaffolding adapter protein Gab2 (Grb2-associated binder) participates in the signaling response evoked by various growth factors and cytokines. Gab2 is … incolink safety netWebApr 11, 2024 · Ph2AX antibody was initially tested on cisplatin-treated and untreated iKras PDAC cells embedded in agarose, and an optimal dilution of 1:400 was determined for the detection of DNA damage cells by IHC staining. Primary and secondary OmniMap HRP-conjugated antibodies were used in 100 μl volumes, for 60 and 16 min, respectively. incolink safety handbookWebFeb 2, 2024 · Antibody buffer and 0.5 μg of antibody were added to samples and incubated overnight at 4°C. After washing beads, 2.5 μl of pAG-NMase was added and incubated for 10 min at RT. After washing, 1 μl of chromatin digest additive was added and incubated for 2 hours at 4°C. Stop buffer and 0.5 ng of Spike-in DNA were then added and incubated for ... incolink payment ratesWebThe validation of phosphosite-specific antibodies requires multiple techniques and tactics to demonstrate their specificity. These antibodies can be used in arrays, flow cytometry, and imaging platforms. The specificity of phosphosite-specific antibodies is vital for their use in proteomics and profiling of disease. less Based on techniques incolink sign inWebPhosphoNET is an open-access, online resource developed by Kinexus Bioinformatics Corporation to foster the study of cell signalling systems to advance biomedical research in academia and industry. PhosphoNET is the worlds largest repository of known and predicted information on human phosphorylation sites, their evolutionary conservation, the ... incolink portable sick leaveWebby a recent study in which phospho-motif speciﬁc antibodies were used to enrich for peptides containing pS/pT-Q, with the goal of identifying substrates of SQ/TQ-directed kinases (e.g., ... including cell line and phosphospeciﬁc-versus-phosphomotif antibody differences. The phosphorylation sites identiﬁed in both data sets are of incolink phone number